Ficoll™ density gradient media tutorial: How to isolate mononuclear cells
2:26
YouTubeCytiva
Ficoll™ density gradient media tutorial: How to isolate mononuclear cells
A step-by step guide on how to best isolate mononuclear cells from human blood. Hints and tips of using Ficoll density gradient centrifugation media. Chapters: 00:00 Introduction 00:14 Add Ficoll to Tube 00:26 Dilute Blood in PBS + Serum 00:44 Prepare 25 ml Blood Sample 00:55 Layer Blood onto Ficoll Carefully 01:17 Centrifuge Setup (400 g, 30 ...
32.1K viewsJan 7, 2021
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